A new method was developed to allow direct visualization of damaged sites on individual DNA molecules. Fluorescence in situ hybridization on extended DNA molecules was modified to detect a single abasic site. Abasic sites were specifically labeled with a biotinylated aldehyde-reactive probe and fluorochrome-conjugated streptavidin. The light emitted by a single fluorochrome–DNA complex was calibrated. The number of abasic sites on the DNA molecule was estimated by counting each fluorochrome–DNA complex. The present study directly visualized and characterized the abasic sites of single DNA molecules.
How to translate text using browser tools
1 August 2002
Direct Visualization of Abasic Sites on a Single DNA Molecule Using Fluorescence Microscopy
Tamaki Hirose,
Toshio Ohtani,
Hiroshi Muramatsu,
Atsushi Tanaka
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
Photochemistry and Photobiology
Vol. 76 • No. 2
August 2002
Vol. 76 • No. 2
August 2002